Fig. 1. Differential expression of Maxi-Cl channel activity between mouse mammary C127 cells and neuroblastoma C1300 cells. A. Maxi-Cl currents recorded in excised inside-out patches from C127 cells, but not from C1300 cells, upon application of an alternating pulse from 0 to ±25 mV (protocol is shown at the top of the traces). In C1300 cells, even after treatment with 10 μM tamoxifen (≥10 min) added to the bath (intracellular) and the pipette (extracellular) solution to mimic conditions previously reported to activate large-conductance anion channel currents in C1300 cells [22]. B. Mean patch currents recorded from C127 cells and C1300 cells in the absence and presence of tamoxifen. Each column represents the mean ± SEM (vertical bar).